Catalog Number - 68040-15P-3M

Cogent NP Amino Wide Pore is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds and larger molecules that are often difficult to retain using conventional reversed-phase columns. Manufactured on high-purity 300 Å wide pore silica with a propyl amino bonded phase, it provides excellent selectivity for complex carbohydrates, sugars, organic acids, and other highly polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC separations involving larger molecular species.

68040-15P-3M is an analytical HPLC column packed with 10 µm NP Amino Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 150 mm format. The 150 mm length provides enhanced resolution and peak capacity compared to shorter columns, making it well suited for more demanding separations. The 10 µm particle size supports higher flow rates and lower operating backpressure, while the wide pore structure improves accessibility for larger molecules. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on analyte properties, mobile phase composition, and instrument configuration.

This column is particularly effective for complex sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other highly polar molecules. The wide pore design provides improved performance for larger analytes in both Normal Phase and HILIC applications. As with all amino phases, this column is suitable for HILIC applications when enhanced retention of polar compounds is required.

Key Benefits

• NP Amino Wide Pore stationary phase

• USP L8 equivalent chemistry

• Propyl amino bonded phase

• 300 Å wide pore silica

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Enhanced retention of polar compounds

• Improved accessibility for larger molecules

• Low operating backpressure with 10 µm particles

• Increased resolving power for complex separations

Typical Applications

• USP L8 methods

• HILIC separations

• Normal Phase chromatography

• Complex sugar analysis

• Carbohydrate analysis

• Organic acid analysis

• Carboxylic acid analysis

• Food and beverage testing

• Pharmaceutical analysis

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: NP Amino Wide Pore

• USP Classification: L8

• Chromatography Mode: Normal Phase / HILIC

• Bonded Phase: Propyl Amino

• Endcapping: Not Endcapped

• Particle Size: 10 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Packed and shipped in Normal Phase solvents

• Suitable for both Normal Phase and HILIC chromatographic modes

• Wide pore design improves access for larger molecules and complex carbohydrates

• Excellent retention of sugars, carbohydrates, and organic acids

• Longer column length provides improved resolution and peak capacity

• Frequently used for method development and preparative chromatography scouting

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